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Table of Contents > Health Dictionary > Fluorescent antibody technique

Fluorescent antibody technique

A technique for antigen using a fluorescent antibody, usually performed by one of two methods: direct, immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe or other), the resulting antigen-antibody complex located by fluorescence microscopy; or indirect, unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex is labeled with fluorescein-conjugated antiimmunoglobulin antibody with the resulting triple complex located by fluorescence microscopy.
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